Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Article Title: Yeast transformation stress, together with loss of Pah1, phosphatidic acid phosphatase, leads to Ty1 retrotransposon insertion into the INO4 gene.

doi: 10.1096/fj.201901811RR

Figure Lengend Snippet: FIGURE 1 pah1∆ cells obtained from a yeast deletion collection showed significant decreases in the phospholipid synthetic enzymes. A, A schematic diagram showing phospholipid synthetic pathways in yeast. B, Heavy membrane fractions prepared from wild-type and Pah1-lacking cells were incubated in the presence of 14C-serine as described in the materials and methods for the indicated time periods. Phospholipids were extracted from the samples and analyzed by TLC and radioimaging. C, 4, 8, and 12 µL of the indicated membrane fractions solubilized in SDS sample buffer to a protein concentration of 2 mg/mL were subjected to SDS-PAGE and analyzed by Western blotting using the indicated antibodies

Article Snippet: Phospholipids dissolved in chloroform were spotted onto a TLC plate (Macherey-Nagel: 810123), which had been dried in 100°C oven after soaking with 1.8% Boric Acid for 5 minutes.

Techniques: Membrane, Incubation, Protein Concentration, SDS Page, Western Blot

Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Article Title: Yeast transformation stress, together with loss of Pah1, phosphatidic acid phosphatase, leads to Ty1 retrotransposon insertion into the INO4 gene.

doi: 10.1096/fj.201901811RR

Figure Lengend Snippet: FIGURE 3 Phospholipid profiles in pah1∆ and pah1∆ cells. A, Total phospholipids prepared from whole wild-type, pah1∆, and pah1*∆ cells with or without the PAH1-encoding plasmid were separated by TLC and analyzed by radioimaging. B, Relative amounts of each phospholipid to total phospholipids were shown Error bars represent standard errors (n = 3)

Article Snippet: Phospholipids dissolved in chloroform were spotted onto a TLC plate (Macherey-Nagel: 810123), which had been dried in 100°C oven after soaking with 1.8% Boric Acid for 5 minutes.

Techniques: Plasmid Preparation

Journal:

Article Title: Measurement of IgG antibodies to Chlamydia trachomatis by commercial enzyme immunoassays and immunofluorescence in sera from pregnant women and patients with infertility, pelvic inflammatory disease, ectopic pregnancy, and laboratory diagnosed Chlamydia psittaci / Chlamydia pneumoniae infection

doi:

Figure Lengend Snippet: Specificity of the commercial enzyme immunoassays (EIAs) for detecting Chlamydia trachomatis antibody when tested against a panel of Chlamydia psittaci/Chlamydia pneumoniae antibody positive sera as diagnosed by whole cell immunofluorescence assay

Article Snippet: Sera for testing were diluted 1/64 in DELFIA assay buffer (Wallac Oy, Turku, Finland) and 100 μl was loaded on to Genzyme Virotech C trachomatis coated microtitre plates (Genzyme Virotech).

Techniques: Enzyme Immunoassay, Immunofluorescence

Journal: Frontiers in Plant Science

Article Title: Molecular Studies of the Protein Complexes Involving Cis -Prenyltransferase in Guayule ( Parthenium argentatum ), an Alternative Rubber-Producing Plant

doi: 10.3389/fpls.2019.00165

Figure Lengend Snippet: Isoprene product separations by reverse phase thin layer chromatography. Microsomes were prepared from the yeast strains selected on 5FOA in , and in vitro enzyme assays were performed using the microsomes and 14 C-isopentenyl diphosphate, followed by TLC (C18 reverse-phase silica) of extracted cis -polyisoprene products using a solvent mixed with acetone and water (39:1), and phosphorimager analysis. (A) The cis -polyisoprene product profiles from yeast RER2 as a size calibration standard. (B) The cis -polyisoprene products from PaCPT/PaCBPs are identical to one another and slightly shorter than those from the RER2-containing microsomes. S indicates the starting point of separation and SF indicates solvent front. R f values and carbon numbers are shown.

Article Snippet: The concentrated products were spotted on a C18 reverse-phase silica plate (Analtech cat. P90011, 20 × 20 cm) and separated by acetone/water (39:1, v/v) mobile phase.

Techniques: Thin Layer Chromatography, In Vitro, Solvent